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Thesis Defense | Reinier Prosee | Centromere Dynamics in the C. Elegan…

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작성자 Jean-Paul Toone… 작성일20-11-26 00:00 조회0회 댓글0건

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Friday, November 20th Reinier Prosee obtained his PhD in Molecular Biology, marking the end of his time in Geneva. The title of his presentation is: Bridging Generations: Centromere Dynamics in the C. Elegans Germ Line.


Trans-generational inheritance of centromere identity requires the CENP-A N-terminal tail in the C. elegans maternal germ line.Centromere protein A (CENP-A) is a histone H3 variant that defines centromeric chromatin and is essential for centromere function. In most eukaryotes CENP-A-containing chromatin is epigenetically maintained, and centromere identity is inherited from one cell cycle to the next. In the germ line of the holocentric nematode Caenorhabditis elegans, this inheritance cycle is disrupted. CENP-A is removed at the mitosis-to-meiosis transition and is established de novo on chromatin during diplotene of meiosis I. Here we show that the N-terminal tail of CENP-A is required for the de novo establishment of centromeres, but dispensable for centromere maintenance during embryogenesis. Worms homozygous for a CENP-A tail deletion maintain a functional centromere during development, but give rise to inviable offspring because they fail to re-establish centromeres in the maternal germ line. We identify the N-terminal tail of CENP-A as a critical domain for the interaction with the conserved kinetochore protein KNL-2, and argue that this interaction plays an important role in setting centromere identity in the germ line. We conclude that centromere establishment and maintenance are functionally distinct in C. elegans.Read more about the abstract and results: https://www.biorxiv.org/content/10.1101/2020.10.05.325985v1.full

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